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      RiPPMiner-Genome: A Bioinformatics Resource for Genome Mining of RiPPs

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BENCHMARKING RESULTS FOR RiPPMINER-GENOME

Correct identification of RiPP BGC/modifying enzymes and prediction of RiPP class could be done in 99% of cases. In more than 70% of cases the prediction corresponding to the correct precursor peptide, cleavage site and cross-link pattern was ranked among top three.

RiPP ClassBGC/GENOME Test SetCorrectly Predicted BGCCorrect Modification Ezyme PredictionCorrectly Predicted RiPP-Precursor PeptideCorrect Leader-Cleavage PredictionCorrect Cross-links Prediction
Lanthipeptide100100100656558
Lassopeptide545454333232
Thiopeptide444343282828
Cyanobactin262626191919
LAPs9994NANA
Glycocin1212123NANA
Head-To-Tail cyclized Peptide9995NANA
Linardin5551NANA
Bottromycin3221NANA

BENCHMARKING RESULTS FOR RiPP PRECURSOR PREDICTION FOR LANTHIPEPTIDE,
THIOPEPTIDE, LASSOPEPTIDE & CYANOBACTIN


BENCHMARKING RESULTS FOR LANTHIPEPTIDE MODIFIED RESIDUES PREDICTION

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Method: Support Vector Machine (SVM)
False Positive Rate (FPR %)True Positive Rate (TPR %)
1078














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The Machine Learning (ML) Model was trained on 49 Lanthipeptides and was Tested on the remaining dataset of 49 Lanthipeptides in the Blind Test. The result shows here are the percent Accuracy for each of these 49 Lanthipeptides. The % Accuracy was calculated by counting the number of correctly predicted Modification States of Ser/Thr/Cys Residues by ML Model divided by total number of such residues present in the Core region of each Lanthipeptide. The average % Accuracy was 76%.